LONG Teng rui, ZHANG Zhao, ZHUAN Rui xin. Biodegradation of Phenol by a Novel Isolated Bacterium Pseudomonas sp. CN 6[J]. Journal of Civil and Environmental Engineering, 2010, 32(5): 82-87.
DOI:
LONG Teng rui, ZHANG Zhao, ZHUAN Rui xin. Biodegradation of Phenol by a Novel Isolated Bacterium Pseudomonas sp. CN 6[J]. Journal of Civil and Environmental Engineering, 2010, 32(5): 82-87. DOI: 10.11835/j.issn.1674-4764.2010.05.015.
Biodegradation of Phenol by a Novel Isolated Bacterium Pseudomonas sp. CN 6
从台湾某纺织厂污泥中分离得到一株能够将苯酚作为唯一碳源和能源生长的细菌。根据其形态和生理生化反应结合16S rDNA测定分析,该菌被鉴定为假单胞杆菌(Pseudomonas sp.)。降解特性研究表明,该菌降解苯酚的最适pH和温度分别为8.5和30℃,在此条件下,浓度高达500 mg/L的苯酚能被该菌在17 h内将完全降解。在动力学研究中,由于高浓度苯酚对CN 6菌株的降解过程存在底物抑制现象,故采用Haldane非竞争性底物抑制模型,计算确定了模型参数μmax、KS和KI分别为0.452 h 1、28.617 mg/L、782.4 mg/L,该动力学方程对实验数据能很好拟合。
Abstract
A strain that have capable of metabolizing phenol as a sole source of carbon and energy sources was isolated from an sludge collected from textile chemical plant in Taiwan. It was identified as Pseudomonas sp. based on its morphology
physiological
biochemical characteristics with an automatic biometrical system and the 16S rDNA sequence analysis. It is shown that the biodegradation of phenol was optimized at culture medium pH 8.5 and 30℃.Under the condition
the strain degraded phenol completely within 17h even though the initial phenol level was high to 500 mg/L. With the study of dynamic growth kinetics of suspended cells
the value of μmax
KS and KI were found to be 0.452 h-1
28.617 mg/L
782.4 mg/L for strain CN 6 degrading phenol using Haldane's inhibition kinetic models.