Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on Phosphorus Accumulating Characteristics

REN Liping; ZHANG Zhi; TANG Yun

doi:10.11835/j.issn.1674-4764.2012.03.025

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Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on Phosphorus Accumulating Characteristics

更新时间：2023-11-28

- Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on Phosphorus Accumulating Characteristics
- Journal of Civil and Environmental Engineering Vol. 34, Issue 3, Pages: 146-150(2012)
- 作者机构：
  
  1. 重庆大学城市建设与环境工程学院,重庆,400030
  2. 西华师范大学生命科学学院,四川,南充,637002
- 作者简介：
- 基金信息：
- DOI：10.11835/j.issn.1674-4764.2012.03.025
  CLC：
- Published：2012
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REN Liping, ZHANG Zhi, TANG Yun. Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on Phosphorus Accumulating Characteristics[J]. Journal of Civil and Environmental Engineering, 2012, 34(3): 146-150.
DOI：

REN Liping, ZHANG Zhi, TANG Yun. Screening and Identification of Phosphate Accumulating Bacteria with Fe (Ⅲ) as Electronic Acceptor and its Effects on Phosphorus Accumulating Characteristics[J]. Journal of Civil and Environmental Engineering, 2012, 34(3): 146-150. DOI： 10.11835/j.issn.1674-4764.2012.03.025.

摘要

结合平板分离法、poly-P和PHB染色以及柠檬酸铁还原实验，从污水处理厂活性污泥中分离获得一株以Fe(Ⅲ)为电子受体厌氧聚磷的聚磷菌株AP3

其16S rDNA与Pseudomonas mosselii ATCCBAA-99的同源性为99%。菌株AP3于柠檬酸铁液体培养基和无铁液体培养基中厌氧避光、27 ℃静置培养，在含有Fe(Ⅲ)的培养基中比在无Fe(Ⅲ)的培养基中生长好；菌株AP3在无Fe(Ⅲ)的培养基中，表现出典型的厌氧释磷特征，释磷量为3.68 mg·L-1；在含有Fe(Ⅲ)的培养基中，先厌氧释磷，释磷量为6.62 mg·L-1， 10 h之后开始厌氧聚磷，聚磷量为5.89 mg·L-1。菌株AP3能以Fe(Ⅲ)为电子受体，具有厌氧聚磷特征。

Abstract

A phosphorus accumulation organism strain AP3 with Fe (Ⅲ) as electronic acceptor was screened from activated sludge through such processes as dilution plate method

poly P

PHB staining and ferric citrate reduction. Its 16S rDNA is identical to that of Pseudomonas mosseliisp ATCCBAA 99 at 99% level. By comparing the AP3 being cultured in ferric citrate fluid medium and the one in non ferric fluid medium at 27 ℃

it was found that ferric citrate fluid medium was beneficial for the AP3. AP3 cultured in non ferric fluid medium showed significant anaerobic and phosphorus releasing characteristic

the phosphorus releasing amount of which was 3.68 mg·L-1. AP3 cultured in ferric fluid medium was also characterized by anaerobic and phosphorus releasing property at the first stage

the phosphorus releasing amount of which was 662 mg·L-1，which was higher than the former; And at the second stage which started from the 10h

it was characterized by anaerobic and phosphorus accumulating property

the phosphorus accumulating amount of which was 5.89 mg·L-1. Fe (Ⅲ) is the electron acceptor of AP3 which shows typical anaerobic and phosphorus absorbing property.

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