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Screening of 17β-estradiol degradation microorganisms and the influence of environmental factors
- Journal of Civil and Environmental Engineering Vol. 44, Issue 6, Pages: 201-208(2022)
- 作者机构：
昆明理工大学 环境科学与工程学院，昆明 650500
- 作者简介：
brief:XIE Wenxiao (1996- ), main research interests: water environment pollution prevention and control research, E-mail: 549404391@qq.com.
HUANG Bin（corresponding author）， PhD， professor， doctorial supervisor， E-mail： huangbin@kust.edu.cn.
- 基金信息：National Natural Science Foundation of China(41761092)
DOI：10.11835/j.issn.2096-6717.2021.264
CLC： X172;X524Received：01 September 2021，
Published：30 December 2022
- 稿件说明：
移动端阅览
谢文骁,来超超,黄斌等.17β-雌二醇降解微生物筛选及环境因素的影响[J].土木与环境工程学报,2022,44(06):201-208.
XIE Wenxiao,LAI Chaochao,HUANG Bin,et al.Screening of 17β-estradiol degradation microorganisms and the influence of environmental factors[J].Journal of Civil and Environmental Engineering,2022,44(06):201-208.
谢文骁,来超超,黄斌等.17β-雌二醇降解微生物筛选及环境因素的影响[J].土木与环境工程学报,2022,44(06):201-208. DOI： 10.11835/j.issn.2096-6717.2021.264.
XIE Wenxiao,LAI Chaochao,HUANG Bin,et al.Screening of 17β-estradiol degradation microorganisms and the influence of environmental factors[J].Journal of Civil and Environmental Engineering,2022,44(06):201-208. DOI： 10.11835/j.issn.2096-6717.2021.264.
摘要
为提高污水中17β-雌二醇（E2）的去除效率，筛选洱海沉积物中E2的优势降解微生物，并在不同环境条件下进行生物吸附和生物降解E2过程的研究。结果表明：大肠杆菌是E2降解的优势菌种，其对E2的生物去除是快速吸附及持续降解的共同作用过程。经大肠杆菌降解3 d后，1.00 mg/L
E2去除率约为70.42%。生物吸附主要受pH值、生物量的限制，在弱碱性（pH=8）环境下吸附效果最佳。在适宜的浓度下，电子供体、H
2
O
2
、腐殖质和重金属可以有效促进E2的生物降解。当葡萄糖、甲酸钠、H
2
O
2
、腐殖质、Zn
2+
和Cu
2+
浓度分别为40.0 mg/L、10.0 mg/L、3.0 mmol/L、2.0~15.0 mg/L、0.5 mg/L及0.5 mg/L时，E2的生物降解率提高近12.41%~57.47%。由此可见，洱海沉积物中筛选的大肠杆菌具有较好的E2去除能力，但其受众多环境因素的影响，通过调节合适的环境条件可极大程度地促进E2的生物去除效率。
Abstract
In order to improve the removal efficiency of 17β-estradiol （E2） in wastewater
the predominant microorganisms that degrade E2 in the Erhai Lake sediment were screened
and the biosorption and biodegradation of E2 in different environment were studied in this study. The results showed that
Escherichia coli
(
E. coli
) is the dominant strain of E2 degradation
and its biological removal process of E2 is a combined process of rapid adsorption and continuous degradation. The removal rate of E2 with an initial concentration of 1.00 mg/L was about 70.42% after being degraded by
E. coli
for 3 days. Biosorption is mainly limited by pH value and biomass
and the highest adsorption efficiency was obtained in a weakly alkaline environment (pH=8). Electron donors
hydrogen peroxide
humus and heavy metals can effectively promote the biodegradation of E2 at appropriate concentrations. When the concentrations of glucose
sodium formate
H
2
O
2
humus
Zn
2+
and Cu
2+
was 40 mg/L
10 mg/L
3 mmol/L
2-15 mg/L
0.5 mg/L and 0.5 mg/L
respectively
the biodegradation efficiency of E2 increased by 12.41%-57.47%. Draw a conclusion from the above results
the
E. coli
screened from the Erhai Lake sediment exhibits excellent E2 removal ability
but this process is affected by many environmental factors
and the removal efficiency of E2 can be greatly promoted by adjusting appropriate environmental conditions.
关键词
Keywords
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