Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines

WANG Yu; JIN Pengkang; REN Tong; SHI Xuan; JIN Xin

doi:10.11835/j.issn.2096-6717.2022.063

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Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines

Environmental Engineering | 更新时间：2023-05-25

- Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines
- Journal of Civil and Environmental Engineering Vol. 45, Issue 3, Pages: 196-204(2023)
- 作者机构：
  
  1.西安建筑科技大学环境与市政工程学院，西安 710055
  2.西安交通大学人居环境与建筑工程学院，西安 710049
- 作者简介：
  
  WANG Yu (1997- ), main research interest: sewage microbial treatment, E-mail: 876510091@qq.com.
  JIN Pengkang （corresponding author）， professor， doctorial supervisor， E-mail： pkjin@hotmail.com.
- 基金信息：
  
  National Natural Science Foundation of China(52070151;51708443);National Key R&D Program(2019YFB2103000);Shanxi Provincial Key R&D Program(2021ZDLSF05-06;2019ZDLNY01-08);Shanxi Provincial Science and Technology Innovation Leading Program(2020CGXNG-021)
- DOI：10.11835/j.issn.2096-6717.2022.063
  CLC： X703.1;X172
- Received：25 February 2022，
  
  Published：25 June 2023
- 稿件说明：
移动端阅览
WANG Yu, JIN Pengkang, REN Tong, et al. Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines[J]. Journal of Civil and Environmental Engineering, 2023, 45(3): 196-204.
DOI：

WANG Yu, JIN Pengkang, REN Tong, et al. Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines[J]. Journal of Civil and Environmental Engineering, 2023, 45(3): 196-204. DOI： 10.11835/j.issn.2096-6717.2022.063.

摘要

从稳定运行的厌氧/好氧/缺氧序批式反应器（A/O/A-SBR）中筛选分离得到3株高效除磷细菌，通过形态观察、生理生化试验及16S rRNA基因序列分析进行物种分析，确定3株细菌分别为不动杆菌属（

Acinetobacter

）、克雷伯菌属（

Klebsiella

）和肠杆菌属（

Enterobacter

），NCBI保存登录号分别为OL519151、OL519152、OL519153。以pH值、温度及碳源种类作为影响因子，分析细菌生长特性和除磷率，同时分析无机磷和有机磷在细菌胞内、溶解性微生物产物（SMP）及胞外聚合物（EPS）中的分布转化规律。结果表明：在最佳环境条件下，

Acinetobacter

sp. PK01、

Klebsiella

sp. PK02和

Enterobacter

sp. PK03菌株的最高除磷率分别达到89.4%、85.43%和76.95%。其中，

Acinetobacter

对环境中磷的去除主要依靠吸收胞外无机磷，并以多聚磷酸盐的形式储存于体内，该途径去除了基质中54.93%的磷；

Klebsiella

对环境中磷的去除主要依靠EPS合成和吸附作用，该途径去除了基质中47.18%的磷；

Enterobacter

主要依靠多聚磷酸盐和EPS的合成作用，去除了基质中48.32%的磷。

Abstract

In the present study

three strains of high-efficiency phosphorus removal bacteria were screened and isolated from a stable anaerobic/aerobic/anoxic sequencing batch reactor (A/O/A-SBR). The species were identified by morphological observation

physiological and biochemical tests and 16S rRNA sequence analysis. The three strains of bacteria were identified as

Acinetobacter

Klebsiella

and

Enterobacter

.The NCBI preservation accession numbers were OL519151

OL519152 and OL519153

respectively. The effects of pH value

temperature and the carbon source on cell grow and phosphorus removal were explored. Meanwhile

the distribution and transformation of inorganic phosphorus and organic phosphorus in bacterial cells

soluble microbial products (SMP) and extracellular polymers (EPS) were also investigated. The results show that the highest phosphorus removal efficiency of

Acinetobacter

sp. PK01

Klebsiella

sp. PK02 and

Enterobacter

sp. PK03 were 89.4%

85.43% and 76.95%

respectively

based on the optimal condition. The removal of phosphorus from the environment by

Acinetobacter

mainly relied on the absorption of extracellular inorganic phosphorus and stored in the body as polyphosphate

removing 54.93% of phosphorus from the matrix. The removal of phosphorus by

Klebsiella

mainly relied on EPS synthesis and adsorption. This route removed 47.18% of phosphorus from the matrix.

Enterobacter

removed 48.32% of phosphorus

which mainly depend on the synthesis of polyphosphate and EPS.

关键词

Keywords

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