Effect of alum content on soybean urease solution and its biocementation

LAI Hanjiang; LUO Kaiwei; CUI Mingjuan; LIU Hui; SHI Jiaxuan; ZHONG Yiping; LIN Chifeng

doi:10.11835/j.issn.2096-6717.2024.116

您当前的位置：

首页 >

文章列表页 >

Effect of alum content on soybean urease solution and its biocementation

Civil Engineering | 更新时间：2026-03-30

- Effect of alum content on soybean urease solution and its biocementation
- Journal of Civil and Environmental Engineering Vol. 48, Issue 2, Pages: 1-7(2026)
- 作者机构：
  
  1.福州大学，紫金地质与矿业学院，福州 350108
  2.福州大学，土木工程学院，福州 350108
  3.智能环境岩土工程福建省闽台科技合作基地，福州 350116
- 作者简介：
  
  LAI Hanjiang (1987- ), professor, doctorial supervisor, main research interests: basic theory and technology development of biogeotechnics, E-mail: laihj@fzu.edu.cn.
  CUI Mingjuan （corresponding author）， PhD， associate professor， E-mail： cuimj@fzu.edu.cn.
- 基金信息：
  
  National Natural Science Foundation of China(42477160;52178319;52108307;51708243);Natural Science Foundation of Fujian Province(2022J05020;2022J05127)
- DOI：10.11835/j.issn.2096-6717.2024.116
  CLC： TU472.99
- Received：19 July 2024，
  
  Published：25 April 2026
- 稿件说明：
移动端阅览
赖汉江,罗开伟,崔明娟等.明矾含量对豆酶液及其生物固化效果的影响[J].土木与环境工程学报,2026,48(02):1-7.

LAI Hanjiang,LUO Kaiwei,CUI Mingjuan,et al.Effect of alum content on soybean urease solution and its biocementation[J].Journal of Civil and Environmental Engineering,2026,48(02):1-7.
赖汉江,罗开伟,崔明娟等.明矾含量对豆酶液及其生物固化效果的影响[J].土木与环境工程学报,2026,48(02):1-7. DOI： 10.11835/j.issn.2096-6717.2024.116.

LAI Hanjiang,LUO Kaiwei,CUI Mingjuan,et al.Effect of alum content on soybean urease solution and its biocementation[J].Journal of Civil and Environmental Engineering,2026,48(02):1-7. DOI： 10.11835/j.issn.2096-6717.2024.116.

摘要

基于自提取豆酶液诱导碳酸钙晶体沉积的岩土体改良技术具有经济、绿色、环境友好等诸多优点，但其较高的浊度易导致生物灌浆过程中发生生物堵塞现象，使得生物固化效果较差。采用含明矾的溶剂提取豆酶液

以降低其浊度；通过豆酶液提取试验、溶液试验、砂柱固化试验并结合宏微观测试，研究不同明矾含量对豆酶液浊度、脲酶活性及生物固化效果的影响。结果表明：添加适量的明矾（如100 g/L的豆粉添加3.0 g/L的明矾）能显著降低豆酶液的浊度，且不影响其脲酶活性，但过量的明矾会导致脲酶活性降低甚至丧失。降低豆酶液浊度有利于改善其生物固化的均匀性，但对所诱导沉积碳酸钙晶体的类型及结晶度并无明显影响。

Abstract

Enzyme induced carbonate precipitation (EICP) technique for soil improvement using self-extracted soybean urease solution to induce calcium carbonate precipitation has various advantages such as economic

ecological and environmental considerations. However

the high turbidity of the self-extracted soybean urease solution easily leads to bioclogging during the biogrouting process

resulting in poor biocementation. In this study

an alum-containing solvent was used for the extraction of soybean urease solution to reduce its turbidity. Incorporating soybean urease extraction

solution tests

and sand column solidification with macro- and micro-scale tests were carried out to investigate the effects of alum content on turbidity and biocementation of the extracted soybean urease solution. The results indicate that the addition of an appropriate amount of alum (e.g.

3.0 g/L for 100 g/L soybean powder content) can effectively reduce the turbidity of the self-extracted soybean urease solution without affecting its urease activity. However

excessive amount of alum may lead to a reduction or even loss of urease activity. The use of a low-turbidity soybean urease solution is beneficial in improving the uniformity of its biocementation

without an apparent effect on the morphology and crystallinity of the precipitated calcium carbonate crystals.

关键词

Keywords

references

刘汉龙 , 肖鹏 , 肖杨 , 等 . MICP胶结钙质砂动力特性试验研究 [J ] . 岩土工程学报 , 2018 , 40 ( 1 ): 38 - 45 .

LIU H L , XIAO P , XIAO Y , et al . Dynamic behaviors of MICP-treated calcareous sand in cyclic tests [J ] . Chinese Journal of Geotechnical Engineering , 2018 , 40 ( 1 ): 38 - 45 . (in Chinese)

LIU Y , GAO Y F , HE J , et al . An experimental investigation of wind erosion resistance of desert sand cemented by soybean-urease induced carbonate precipitation [J ] . Geoderma , 2023 , 429 : 116231 .

WANG H X , SUN X H , MIAO L C , et al . Garlic extract addition for soil improvement at various temperatures using enzyme-induced carbonate precipitation (EICP) method [J ] . Journal of Rock Mechanics and Geotechnical Engineering , 2023 , 15 ( 12 ): 3230 - 3243 .

CUI M J , LAI H J , WU S F , et al . Comparison of soil improvement methods using crude soybean enzyme, bacterial enzyme or bacteria-induced carbonate precipitation [J ] . Géotechnique , 2024 , 74 ( 1 ): 18 - 26 .

YASUHARA H , NEUPANE D , HAYASHI K , et al . Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation [J ] . Soils and Foundations , 2012 , 52 ( 3 ): 539 - 549 .

ARAB M G , ROHY H , ZEIADA W , et al . One-phase EICP biotreatment of sand exposed to various environmental conditions [J ] . Journal of Materials in Civil Engineering , 2021 , 33 ( 3 ): 04020489 .

HOANG T , ALLEMAN J , CETIN B , et al . Sand and silty-sand soil stabilization using bacterial enzyme-induced calcite precipitation (BEICP) [J ] . Canadian Geotechnical Journal , 2019 , 56 ( 6 ): 808 - 822 .

HE J , GAO Y F , GU Z X , et al . Characterization of crude bacterial urease for CaCO 3 precipitation and cementation of silty sand [J ] . Journal of Materials in Civil Engineering , 2020 , 32 ( 5 ): 04020071 .

CUI M J , LAI H J , HOANG T , et al . One-phase-low-pH enzyme induced carbonate precipitation (EICP) method for soil improvement [J ] . Acta Geotechnica , 2021 , 16 ( 2 ): 481 - 489 .

DILRUKSHI R A N , WATANABE J , KAWASAKI S . Sand cementation test using plant-derived urease and calcium phosphate compound [J ] . Materials Transactions , 2015 , 56 ( 9 ): 1565 - 1572 .

SHU S , YAN B Y , GE B , et al . Factors affecting soybean crude urease extraction and biocementation via enzyme-induced carbonate precipitation (EICP) for soil improvement [J ] . Energies , 2022 , 15 ( 15 ): 5566 .

YAN B Y , ZHOU Y D , LI C , et al . Modified SICP method to mitigate the effect of bio-clogging by excess protein from soybean crude urease extracts for biocementation process [J ] . Acta Geotechnica , 2023 , 18 ( 9 ): 5047 - 5062 .

LAI H J , CUI M J , WU S F , et al . Extraction of crude soybean urease using ethanol and its effect on soil cementation [J ] . Soils and Foundations , 2023 , 63 ( 3 ): 101300 .

WENG Y J , ZHENG J J , LAI H J , et al . Biomineralization of soil with crude soybean urease using different calcium salts [J ] . Journal of Rock Mechanics and Geotechnical Engineering , 2024 , 16 ( 5 ): 1788 - 1798 .

LAI H J , CUI M J , CHU J . Effect of pH on soil improvement using one-phase-low-pH MICP or EICP biocementation method [J ] . Acta Geotechnica , 2023 , 18 ( 6 ): 3259 - 3272 .

CUI M J , ZHENG J J , ZHANG R J , et al . Influence of cementation level on the strength behaviour of bio-cemented sand [J ] . Acta Geotechnica , 2017 , 12 ( 5 ): 971 - 986 .

LAI H J , DING X Z , CUI M J , et al . Factors affecting the effectiveness of biocementation of soil [J ] . Biogeotechnics , 2024 , 2 ( 3 ): 100087 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Extraction of potato urease and improvement of aeolian sand based on EICP Technology

Morphology and structural characteristics of composite flocculant PTA-CPAM and its performance for water purification

Related Author

LI Chi

GAO Yu

WU Huimin

CHU Wenjie

Guocheng Zhu

Weixiong Liao

Yulu Wang

Peng Zhang

Related Institution

School of Civil Engineering;key Lab. of Geological Hazards and Geotechnical Engineering Defense in Sandy and Drought Regions, Inner Mongolia University of Technology

Inner Mongolia Water Conservancy and Hydropower Survey and Design Institute

AI问答

Address：Journals Department, Chongqing University, 174 Shazhengjie, Chongqing 400044, P.R.China Postal code：400044
Tel：+86-23-65111322 Email：xuebao@cqu.edu.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰