FISH技术在污水生物除磷脱氮研究中的应用

颜强; 谢有奎

doi:10.11835/j.issn.1674-4764.2004.06.016

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FISH技术在污水生物除磷脱氮研究中的应用

更新时间：2023-11-28

- FISH技术在污水生物除磷脱氮研究中的应用
- Application of FISH in Biological Removal of Nitrogen and Phosphorus from Wastewater
- 土木与环境工程学报（中英文） 2004年26卷第6期页码：71-73
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- DOI：10.11835/j.issn.1674-4764.2004.06.016
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- 纸质出版：2004
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颜强, 谢有奎. FISH技术在污水生物除磷脱氮研究中的应用[J]. 土木与环境工程学报（中英文）, 2004,26(6):71-73.

YAN Qiang~1, XIE You-kui~2. Application of FISH in Biological Removal of Nitrogen and Phosphorus from Wastewater[J]. Journal of Civil and Environmental Engineering, 2004, 26(6): 71-73.
颜强, 谢有奎. FISH技术在污水生物除磷脱氮研究中的应用[J]. 土木与环境工程学报（中英文）, 2004,26(6):71-73. DOI： 10.11835/j.issn.1674-4764.2004.06.016.

YAN Qiang~1, XIE You-kui~2. Application of FISH in Biological Removal of Nitrogen and Phosphorus from Wastewater[J]. Journal of Civil and Environmental Engineering, 2004, 26(6): 71-73. DOI： 10.11835/j.issn.1674-4764.2004.06.016.

摘要

在简单介绍荧光原位杂交(FISH)的基本原理后，着重讨论了该技术在污水生物除磷、脱氮工艺中的应用现状、特点和前景。尽管污水生物除磷、脱氮工艺的宏观性能与微生物相的关系密切，但因缺乏适宜的检测技术，对工艺系统中微生物类型、数量和结构等了解还很不充分。研究表明：FISH技术能够为除磷菌、硝化菌的识别与再分类提供优越的检测手段；与聚合酶链反应(PCR)等其它技术相结合，可使FISH技术更具生命力。

Abstract

The application of Fluorescence In-situ Hybridization (FISH) to research of biological removal of nitrogen and phosphorous from wastewater was discussed after a brief introduction of its principal theory.Though there exists close relationship between macroscopical performance of biological removal of nitrogen and phosphorous and the state of microorganism

the sort

quantity and structure of microbes inside the wastewater treating system had not been well understood yet because of lack of proper examining technology.The research shows that FISH can provide an excellent examining method for identification and sub-classification of polyphosphate accumulating organisms (PAO) and nitrifying bacteria.Moreover

the combination of FISH with other methods such as polymerase chain reaction (PCR) can make it more powerful.

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