TISTD反应器中的菌群结构及生态变化

叶姜瑜; 丁维; 何强; 孙兴福

doi:10.11835/j.issn.1674-4764.2011.01.025

您当前的位置：

首页 >

文章列表页 >

TISTD反应器中的菌群结构及生态变化

更新时间：2023-11-28

- TISTD反应器中的菌群结构及生态变化
- Microbial Community Structure and Ecological Variation in TISTD Reactor
- 土木与环境工程学报（中英文） 2011年33卷第1期页码：147-152
- 作者机构：
  
  重庆大学城市建设与环境工程学院,三峡库区生态环境教育部重点实验室,重庆,400045
- 作者简介：
- 基金信息：
  
  科技部国际合作项目(2007DFA90660);国家水体污染控制与治理科技重大专项(2008ZX07315-003)
- DOI：10.11835/j.issn.1674-4764.2011.01.025
  中图分类号：
- 纸质出版：2011
- 稿件说明：
移动端阅览
叶姜瑜, 丁维, 何强, 等. TISTD反应器中的菌群结构及生态变化[J]. 土木与环境工程学报（中英文）, 2011,33(1):147-152.

YE Jiang yu, DING Wei, HE Qiang, et al. Microbial Community Structure and Ecological Variation in TISTD Reactor[J]. Journal of Civil and Environmental Engineering, 2011, 33(1): 147-152.
叶姜瑜, 丁维, 何强, 等. TISTD反应器中的菌群结构及生态变化[J]. 土木与环境工程学报（中英文）, 2011,33(1):147-152. DOI： 10.11835/j.issn.1674-4764.2011.01.025.

YE Jiang yu, DING Wei, HE Qiang, et al. Microbial Community Structure and Ecological Variation in TISTD Reactor[J]. Journal of Civil and Environmental Engineering, 2011, 33(1): 147-152. DOI： 10.11835/j.issn.1674-4764.2011.01.025.

摘要

以两相一体式污泥浓缩消化反应器(Two-phase Integrated Sludge Thickening and Digestion reactor

TISTD)为对象

研究了反应器稳定运行期间菌群结构及反应器负荷改变时其内部生态结构的变化。在以30%的投配率稳定运行时

从产酸相和产甲烷相分别取样、培养和分离出20株优势菌属。选取其中6株进行了16S rDNA序列分析

分属于芽孢杆菌属、产甲烷螺菌属和产甲烷球菌属。在投配率分别为10%、20%、30%以及启动期和系统崩溃期5个时期采取10组样品

对其污泥进行DNA提取、纯化和16S rDNA PCR扩增及温度梯度凝胶电泳(Temperature gradient gel electrophoresis

TGGE)电泳检测等。结果表明：中温下反应器中微生物菌群呈多样性分布

生态系统复杂稳定

维持了反应器的正常功能；反应器不同负荷时期污泥DNA提取及扩增效果良好；TGGE图谱分析表明

投配率20%、30%时的群落结构相似度最高

污泥中生物含量丰富

支撑了TISTD在污泥浓缩下良好的消化效果。

Abstract

Based on the sewage treatment device of Two-phase Integrated Sludge Thickening and Digestion reactor (TISTD)

microbial community structure in stable operation and its ecological variation when load changes are investigated. During a stable operation with 30% sludge dosage rate

20 dominant bacterial strains are individually isolated from acid-phase and methane-phase of the reactor. Sequence analysis of 16S rDNA reveals that six of the achieved strains belong to Bacillus

Methanospirillum and Methanococcus respectively. Ten sludge samples are collected separately in 5 periods when the reactor operates with 10%

20% and 30% sludge dosage rate and in the state of started-up and disordered

and followed by DNA extraction

purification

16S rDNA PCR amplification and temperature gradient gel electrophoresis (TGGE). The good quality of DNA extraction and amplification demonstrates that there is an abundant biomass in the sludge under mid-temperature condition. The results show that TISTD reactor has a high biodiversity in microbial communities and is complex and stable in ecosystem structure

which effectively supports the good digestion in sludge thickening.

关键词

Keywords

references

浏览量

815

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据