In the experiment

the denitrifying phosphorus removing bacteria (DPB) was cultivated by high phosphorus and low carbon source influent. It was cultivated successfully by the 2 stage mode for about 100 d. The first stage was about 40 d

which was with the mode of anaerobic/aerobic to enrich the phosphorus accumulating organisms (PAOs). The maximum amounts of the phosphorus release

phosphorus uptake and phosphorus removal were 77.2 mg/L

89.4 mg/L and 250 mg/L respectively. The second stage was 60 d

which was with the mode of anaerobic/anoxic/aerobic. In this stage

the ratio of denitrifying phosphorus uptake amount to the total phosphorus uptake amount had an ascending tendency. The cultivation of nitrifying activated sludge was about 100 d

with the 50 mg/L ammonia removal amount and more than 98.5% ammonia removal efficiency. Nitrification rate fitted zero order kinetic equation

and the specific nitrification rate constant was 0.002 4 h-1. Phosphorus uptake rates fitted first order absorption kinetic equation. The uptake rate constants under aerobic and anoxic conditions were 0.377 and 0.740 L/(g·h-1). The cultivated DPB sludge and nitrifying activated sludge were used in an anaerobic/anoxic/nitrification sequencing batch reactor（A 2N-SBR） process to remove nitrogen and phosphorus. When the concentrations of influent COD

ammonia and phosphorus were 188.0 mg/L

54.8 mg/L and 725 mg/L

the removal efficiencies of COD

ammonia and phosphorus were 935%

76.7% and 94.1% respectively.