3株高效除磷细菌的分离鉴定及磷去除途径分析

王雨; 金鹏康; 任童; 石烜; 金鑫

doi:10.11835/j.issn.2096-6717.2022.063

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3株高效除磷细菌的分离鉴定及磷去除途径分析

环境工程 | 更新时间：2023-05-25

- 3株高效除磷细菌的分离鉴定及磷去除途径分析
- Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines
- 土木与环境工程学报 2023年45卷第3期页码：196-204
- 作者机构：
  
  1.西安建筑科技大学环境与市政工程学院，西安 710055
  2.西安交通大学人居环境与建筑工程学院，西安 710049
- 作者简介：
  
  王雨（1997- ），女，主要从事污水微生物处理研究，E-mail：876510091@qq.com。
  金鹏康（通信作者），男，教授，博士生导师，E-mail：pkjin@hotmail.com。
- 基金信息：
  
  国家自然科学基金(52070151;51708443);国家重点研发计划(2019YFB2103000);陕西省重点研发计划(2021ZDLSF05-06;2019ZDLNY01-08);陕西省科技创新引领计划(2020CGXNG-021)
- DOI：10.11835/j.issn.2096-6717.2022.063
  中图分类号： X703.1;X172
- 收稿：2022-02-25，
  
  纸质出版：2023-06-25
- 稿件说明：
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王雨, 金鹏康, 任童, 等. 3株高效除磷细菌的分离鉴定及磷去除途径分析[J]. 土木与环境工程学报, 2023,45(3):196-204.

WANG Yu, JIN Pengkang, REN Tong, et al. Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines[J]. Journal of Civil and Environmental Engineering, 2023, 45(3): 196-204.
王雨, 金鹏康, 任童, 等. 3株高效除磷细菌的分离鉴定及磷去除途径分析[J]. 土木与环境工程学报, 2023,45(3):196-204. DOI： 10.11835/j.issn.2096-6717.2022.063.

WANG Yu, JIN Pengkang, REN Tong, et al. Isolation and identification of three high efficiency polyphosphate-accumulating organism and analysis of phosphorus removal routines[J]. Journal of Civil and Environmental Engineering, 2023, 45(3): 196-204. DOI： 10.11835/j.issn.2096-6717.2022.063.

摘要

从稳定运行的厌氧/好氧/缺氧序批式反应器（A/O/A-SBR）中筛选分离得到3株高效除磷细菌，通过形态观察、生理生化试验及16S rRNA基因序列分析进行物种分析，确定3株细菌分别为不动杆菌属（

Acinetobacter

）、克雷伯菌属（

Klebsiella

）和肠杆菌属（

Enterobacter

），NCBI保存登录号分别为OL519151、OL519152、OL519153。以pH值、温度及碳源种类作为影响因子，分析细菌生长特性和除磷率，同时分析无机磷和有机磷在细菌胞内、溶解性微生物产物（SMP）及胞外聚合物（EPS）中的分布转化规律。结果表明：在最佳环境条件下，

Acinetobacter

sp. PK01、

Klebsiella

sp. PK02和

Enterobacter

sp. PK03菌株的最高除磷率分别达到89.4%、85.43%和76.95%。其中，

Acinetobacter

对环境中磷的去除主要依靠吸收胞外无机磷，并以多聚磷酸盐的形式储存于体内，该途径去除了基质中54.93%的磷；

Klebsiella

对环境中磷的去除主要依靠EPS合成和吸附作用，该途径去除了基质中47.18%的磷；

Enterobacter

主要依靠多聚磷酸盐和EPS的合成作用，去除了基质中48.32%的磷。

Abstract

In the present study

three strains of high-efficiency phosphorus removal bacteria were screened and isolated from a stable anaerobic/aerobic/anoxic sequencing batch reactor (A/O/A-SBR). The species were identified by morphological observation

physiological and biochemical tests and 16S rRNA sequence analysis. The three strains of bacteria were identified as

Acinetobacter

Klebsiella

and

Enterobacter

.The NCBI preservation accession numbers were OL519151

OL519152 and OL519153

respectively. The effects of pH value

temperature and the carbon source on cell grow and phosphorus removal were explored. Meanwhile

the distribution and transformation of inorganic phosphorus and organic phosphorus in bacterial cells

soluble microbial products (SMP) and extracellular polymers (EPS) were also investigated. The results show that the highest phosphorus removal efficiency of

Acinetobacter

sp. PK01

Klebsiella

sp. PK02 and

Enterobacter

sp. PK03 were 89.4%

85.43% and 76.95%

respectively

based on the optimal condition. The removal of phosphorus from the environment by

Acinetobacter

mainly relied on the absorption of extracellular inorganic phosphorus and stored in the body as polyphosphate

removing 54.93% of phosphorus from the matrix. The removal of phosphorus by

Klebsiella

mainly relied on EPS synthesis and adsorption. This route removed 47.18% of phosphorus from the matrix.

Enterobacter

removed 48.32% of phosphorus

which mainly depend on the synthesis of polyphosphate and EPS.

关键词

Keywords

references

邓荣森 , 郎建 , 王涛 , 等 . 城市污水生物除磷脱氮机理研究探讨 [J]. 重庆建筑大学学报 , 2002 , 24 ( 3 ): 106 - 111 .

DENG R S , LANG J , WANG T , et al . A study on mechanism of biological denitrification and phosphorus removal for urban wastewater [J]. Journal of Chongqing Jianzhu University , 2002 , 24 ( 3 ): 106 - 111 . (in Chinese)

FUHS G W , CHEN M . Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater [J]. Microbial Ecology , 1975 , 2 ( 2 ): 119 - 138 .

王楠 , 蔡曼莎 , 李亚静 , 等 . 运行条件对强化生物除磷颗粒污泥系统性能及微生物群落的影响 [J]. 土木与环境工程学报（中英文） , 2021 , 43 ( 4 ): 202 - 210 .

WANG N , CAI M S , LI Y J , et al . Effect of operation conditions on the performance and microbial community of an enhanced biological phosphorus removal granular sludge system [J]. Journal of Civil and Environmental Engineering , 2021 , 43 ( 4 ): 202 - 210 . (in Chinese)

汪昆平 , 邓荣森 , 李伟民 , 等 . 氧化沟脱氮除磷强化途径 [J]. 重庆建筑大学学报 , 2006 , 28 ( 6 ): 79 - 83 .

WANG K P , DENG R S , LI W M , et al . Approach to the enhancement of removal of phosphorus and nitrogen in oxidation ditch [J]. Journal of Chongqing Jianzhu University , 2006 , 28 ( 6 ): 79 - 83 . (in Chinese)

张园 , 罗固源 , 许晓毅 , 等 . UCT工艺进水COD浓度与C/N对除磷效果的影响 [J]. 环境科学 , 2010 , 31 ( 8 ): 1846 - 1850 .

ZHANG Y , LUO G Y , XU X Y , et al . Effect of the influent COD and C/N ratio on phosphorus removal of UCT system [J]. Environmental Science , 2010 , 31 ( 8 ): 1846 - 1850 . (in Chinese)

ALMOMANI F , BOHSALE R R . Optimizing nutrient removal of moving bed biofilm reactor process using response surface methodology [J]. Bioresource Technology , 2020 , 305 : 123059 .

庄志刚 , 韩永和 , 章文贤 , 等 . 高效聚磷菌 Alcaligenes sp.ED-12菌株的分离鉴定及其除磷特性 [J]. 环境科学学报 , 2014 , 34 ( 3 ): 678 - 687 .

ZHUANG Z G , HAN Y H , ZHANG W X , et al . Isolation, identification and phosphorus-removal characterization of bacteria Alcaligenes sp. strain ED-12 for phosphorus-accumulation [J]. Acta Scientiae Circumstantiae , 2014 , 34 ( 3 ): 678 - 687 . (in Chinese)

FAN Z W , ZENG W , WANG B G , et al . Transcriptional responses of Candidatus Accumulibacter clades to environmental dynamics in enhanced biological phosphorus removal [J]. Bioresource Technology , 2020 , 306 : 123108 .

WU L W , NING D L , ZHANG B , et al . Global diversity and biogeography of bacterial communities in wastewater treatment plants [J]. Nature Microbiology , 2019 , 4 ( 7 ): 1183 - 1195 .

吴云 , 范丙全 , 隋新华 , 等 . 适应菲胁迫的高效聚磷菌筛选及聚磷特性研究 [J]. 环境科学 , 2008 , 29 ( 11 ): 3172 - 3178 .

WU Y , FAN B Q , SUI X H , et al . Screening of two phenanthrene-utilizing and high-effective phosphorus-accumulating bacteria and their effects on phosphorus-accumulating characteristics [J]. Environmental Science , 2008 , 29 ( 11 ): 3172 - 3178 . (in Chinese)

张文艺 , 陈晶 , 邓文 , 等 . 反硝化聚磷菌菌剂种子液制备条件及除磷机理 [J]. 土木建筑与环境工程 , 2014 , 36 ( 6 ): 99 - 105 .

ZHANG W Y , CHEN J , DENG W , et al . Preparation of denitrifying phosphorus accumulating bacterial seed liquid and analysis of phosphorus removal mechanism [J]. Journal of Civil , Architectural & Environmental Engineering, 2014 , 36 ( 6 ): 99 - 105 . (in Chinese)

CLOSE K , MARQUES R , CARVALHO V C F , et al . The storage compounds associated with Tetrasphaera PAO metabolism and the relationship between diversity and P removal [J]. Water Research , 2021 , 204 : 117621 .

REN T , CHI Y L , WANG Y , et al . Diversified metabolism makes novel Thauera strain highly competitive in low carbon wastewater treatment [J]. Water Research , 2021 , 206 : 117742 .

张娟 , 方祥位 , 刘汉龙 , 等 . 石油污染土中微生物的分离鉴定及降解特性 [J]. 土木与环境工程学报(中英文) , 2020 , 42 ( 1 ): 144 - 152 .

ZHANG J , FANG X W , LIU H L , et al . Isolation, identification and degradation characteristics of microorganisms in petroleum contaminated soil [J]. Journal of Civil and Environmental Engineering , 2020 , 42 ( 1 ): 144 - 152 . (in Chinese)

陈亚松 , 金文标 , 闫韫 , 等 . 高效聚磷菌的筛选及其应用 [J]. 净水技术 , 2011 , 30 ( 2 ): 19 - 22 .

CHEN Y S , JIN W B , YAN Y , et al . Screening and application of high efficient phosphate-accumulating organisms (PAOs) [J]. Water Purification Technology , 2011 , 30 ( 2 ): 19 - 22 . (in Chinese)

马放 , 杨菲菲 , 李昂 , 等 . 1株高效反硝化聚磷菌的生物学特性研究 [J]. 环境科学 , 2011 , 32 ( 9 ): 2710 - 2715 .

MA F , YANG F F , LI A , et al . Biological characteristics of denitrifying polyphosphate-accumulating organisms [J]. Environmental Science , 2011 , 32 ( 9 ): 2710 - 2715 . (in Chinese)

蔡天明 , 陈立伟 , 吴守中 , 等 . 1株脱氮除磷菌的筛选及其特性研究 [J]. 环境科学 , 2010 , 31 ( 10 ): 2487 - 2492 .

CAI T M , CHEN L W , WU S Z , et al . Selection of denitrifying phosphorus-removing bacteria and its characteristic [J]. Environmental Science , 2010 , 31 ( 10 ): 2487 - 2492 . (in Chinese)

欧阳彤 , 涂保华 , 李乔 , 等 . 多级AO+潜流湿地对生活污水中的EDCs及常规污染物的去除试验研究 [J]. 土木与环境工程学报(中英文) , 2020 , 42 ( 3 ): 156 - 164 .

OUYANG T , TU B H , LI Q , et al . Experimental study on the removal of EDCs and conventional pollutants indomestic sewage by multi-stage AO + subsurface flow constructed wetland [J]. Journal of Civil and Environmental Engineering , 2020 , 42 ( 3 ): 156 - 164 . (in Chinese)

翟俊 , 李岳 . 微曝气强化人工湿地处理生活污水试验研究 [J]. 土木与环境工程学报(中英文) , 2020 , 42 ( 6 ): 178 - 184 .

ZHAI J , LI Y . The effects of domestic wastewater treatment by micro-aerated hybrid constructed wetland [J]. Journal of Civil and Environmental Engineering , 2020 , 42 ( 6 ): 178 - 184 . (in Chinese)

ZHOU Y , NGUYEN B T , ZHOU C , et al . The distribution of phosphorus and its transformations during batch growth of Synechocystis [J]. Water Research , 2017 , 122 : 355 - 362 .

RODGERS M , WU G X . Production of polyhydroxybutyrate by activated sludge performing enhanced biological phosphorus removal [J]. Bioresource Technology , 2010 , 101 ( 3 ): 1049 - 1053 .

LI H K , ZHONG Y M , HUANG H , et al . Simultaneous nitrogen and phosphorus removal by interactions between phosphate accumulating organisms (PAOs) and denitrifying phosphate accumulating organisms (DPAOs) in a sequencing batch reactor [J]. The Science of the Total Environment , 2020 , 744 : 140852 .

WANG D B , ZHENG W , LIAO D X , et al . Effect of initial pH control on biological phosphorus removal induced by the aerobic/extended-idle regime [J]. Chemosphere , 2013 , 90 ( 8 ): 2279 - 2287 .

ZHANG T , LIU Y , FANG H H P . Effect of pH change on the performance and microbial community of enhanced biological phosphate removal process [J]. Biotechnology and Bioengineering , 2005 , 92 ( 2 ): 173 - 182 .

YUAN C S , WANG B , PENG Y Z , et al . Simultaneous enhanced biological phosphorus removal and semi-nitritation (EBPR-SN) followed by anammox process treating municipal wastewater at seasonal temperatures: From summer to winter [J]. Science of the Total Environment , 2021 , 757 : 144048 .

LASPIDOU C S , RITTMANN B E . A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass [J]. Water Research , 2002 , 36 ( 11 ): 2711 - 2720 .

DIXON M , FLINT S , PALMER J , et al . Analysis of culturable and non-culturable bacteria and their potential to form biofilms in a primary treated dairy wastewater system [J]. Environmental Technology , 2018 , 39 ( 17 ): 2185 - 2192 .

YANG J X , WEI W , PI S S , et al . Competitive adsorption of heavy metals by extracellular polymeric substances extracted from Klebsiella sp. J1 [J]. Bioresource Technology , 2015 , 196 : 533 - 539 .

李雅婷 . 氮磷同化海洋菌-藻共生系统的建立及其在含盐污水处理中的研究 [D]. 济南 : 山东大学 , 2018 .

LI Y T . Establishment of nitrogen-phosphorus assimilation marine bacterial-algal mutualistic system and its application in saline sewage treatment [D]. Jinan : Shandong University , 2018 . (in Chinese)

ZHANG H L , FANG W , WANG Y P , et al . Phosphorus removal in an enhanced biological phosphorus removal process: Roles of extracellular polymeric substances [J]. Environmental Science & Technology , 2013 , 47 ( 20 ): 11482 - 11489 .

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